Hotspot

trnN-GUU-trnR-ACG

IRa · 132868-133510 · 643 bp

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Pi 0.00627

MarkerSeek score 63.10

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 643
variable_sites: 4
pi: 0.00627
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	779-784 bp	1.0	79.2
trnN-GUU-trnR-ACG_p2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	55.0	55.0	58.0	57.7	816-821 bp	1.0	78.6
trnN-GUU-trnR-ACG_p3	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	820-825 bp	1.0	78.4
trnN-GUU-trnR-ACG_p4	CCGTTGTTCCTCAGTAGCTC	TTTTAGGGTACTCCGGGGAA	55.0	50.0	58.0	57.7	833-838 bp	1.0	78.1
trnN-GUU-trnR-ACG_p5	CCGTTGTTCCTCAGTAGCTC	TTTTTAGGGTACTCCGGGGA	55.0	50.0	58.0	57.7	834-839 bp	1.0	78.1