Hotspot

ndhG-ndhI

SSC · 121884-122284 · 401 bp

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Pi 0.00748

MarkerSeek score 58.90

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 401
variable_sites: 3
pi: 0.00748
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	ATGTAGCGGTTCAGGCAAAT	AATCAAATTGCTTTGGGCCG	45.0	45.0	57.9	57.9	500 bp	1.0	78.7
ndhG-ndhI_p2	ATGTAGCGGTTCAGGCAAAT	TTTGGGCCGTTTACCAATGT	45.0	45.0	57.9	58.3	489 bp	1.0	77.1
ndhG-ndhI_p3	AATCATGTAGCGGTTCAGGC	AATCAAATTGCTTTGGGCCG	50.0	45.0	58.3	57.9	504 bp	1.0	77.0
ndhG-ndhI_p4	ATGTAGCGGTTCAGGCAAAT	CTTTGGGCCGTTTACCAATG	45.0	50.0	57.9	57.6	490 bp	1.0	76.6
ndhG-ndhI_p5	ATGTAGCGGTTCAGGCAAAT	CAAATTGCTTTGGGCCGTTT	45.0	45.0	57.9	58.4	497 bp	1.0	76.2