Hotspot

rpl32-trnL-UAG

SSC · 116528-117240 · 713 bp

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Pi 0.0014

MarkerSeek score 59.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 713
variable_sites: 1
pi: 0.0014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGAGGGGATATTGGACAGCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.8	57.9	835-859 bp	1.0	77.5
rpl32-trnL-UAG_p2	AGAGGGGATATTGGACAGCA	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.8	57.8	828-852 bp	1.0	77.0
rpl32-trnL-UAG_p3	AGAGGGGATATTGGACAGCA	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	57.8	57.8	834-858 bp	1.0	77.0
rpl32-trnL-UAG_p4	AGAGGGGATATTGGACAGCA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	57.8	57.8	833-857 bp	1.0	77.0
rpl32-trnL-UAG_p5	AGAGGGGATATTGGACAGCA	GAGCAGCGTGTCTACCAATT	50.0	50.0	57.8	58.3	829-853 bp	1.0	76.3