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rps11-rpl36

LSC · 83608-84197 · 590 bp

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Pi 0.00169

MarkerSeek score 59.50

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 590
variable_sites: 1
pi: 0.00169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	ATTTTACGCGCATGCTTACG	GCGGGGGCGAATTATAGTAA	45.0	50.0	57.9	57.5	722 bp	1.0	75.7
rps11-rpl36_p2	CGTCCATTTTTACGCGAACC	GCGGGGGCGAATTATAGTAA	50.0	50.0	58.1	57.5	692 bp	1.0	75.4
rps11-rpl36_p3	TTGGTATTTTACGCGCATGC	GCGGGGGCGAATTATAGTAA	45.0	50.0	57.8	57.5	727 bp	1.0	75.0
rps11-rpl36_p4	CCCTTTGGTATTTTACGCGC	GCGGGGGCGAATTATAGTAA	50.0	50.0	57.8	57.5	731 bp	1.0	75.0
rps11-rpl36_p5	TTACGCGAACCAATACGTCC	GCGGGGGCGAATTATAGTAA	50.0	50.0	58.4	57.5	707 bp	1.0	73.5