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accD-psaI

LSC · 62285-63010 · 726 bp

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Pi 0.00413

MarkerSeek score 54.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 726
variable_sites: 3
pi: 0.00413
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TTGATTCAATCGTACCGCGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	897 bp	1.0	80.0
accD-psaI_p2	TTGATTCAATCGTACCGCGT	AAGGCACAAAAATGGAGGGT	45.0	45.0	58.0	57.9	864 bp	1.0	79.5
accD-psaI_p3	TTGATTCAATCGTACCGCGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	58.0	57.9	903 bp	1.0	79.4
accD-psaI_p4	TCAATCGTACCGCGTAATCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	892 bp	1.0	79.1
accD-psaI_p5	CAATCGTACCGCGTAATCCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	891 bp	1.0	79.0