Hotspot

psbK-trnQ-UUG

LSC · 57586-58386 · 801 bp

Back to Zollernia

Pi 0.0025

MarkerSeek score 58.40

Primer available yes

Label psbK-trnQ-UUG

Feature Metadata

label_name: psbK-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 801
variable_sites: 2
pi: 0.0025
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-trnQ-UUG_p1	AGGCTTCAGGTAATTTCGCC	GATATGCTCTGGGACGGAAG	50.0	55.0	58.2	57.6	968-969 bp	1.0	74.7
psbK-trnQ-UUG_p2	AGGCTTCAGGTAATTTCGCC	GACCAAAACCCGTTGCCTTA	50.0	50.0	58.2	58.7	926-927 bp	1.0	72.8
psbK-trnQ-UUG_p3	GGCTTCAGGTAATTTCGCCA	GATATGCTCTGGGACGGAAG	50.0	55.0	58.5	57.6	967-968 bp	1.0	72.3
psbK-trnQ-UUG_p4	AGGCTTCAGGTAATTTCGCC	AATGGATATGCTCTGGGACG	50.0	50.0	58.2	57.2	972-973 bp	1.0	71.5
psbK-trnQ-UUG_p5	AGGCTTCAGGTAATTTCGCC	GAATAGCGGGACCAAAACCC	50.0	55.0	58.2	58.9	935-936 bp	1.0	71.1