Hotspot

trnG-UCC-trnS-GCU

LSC · 55656-56555 · 900 bp

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Pi 0.00111

MarkerSeek score 59.40

Primer available yes

Label trnG-UCC-trnS-GCU

Feature Metadata

label_name: trnG-UCC-trnS-GCU
feature_type: igs
region: LSC
length_bp: 900
variable_sites: 1
pi: 0.00111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnS-GCU_p1	GGGGTCCATAGAACGAATCG	CAATCCGACGCTTTAGTCCA	55.0	50.0	57.8	58.0	981 bp	1.0	78.8
trnG-UCC-trnS-GCU_p2	GGGGTCCATAGAACGAATCG	AATCCGACGCTTTAGTCCAC	55.0	50.0	57.8	58.0	980 bp	1.0	78.8
trnG-UCC-trnS-GCU_p3	GGTCCATAGAACGAATCGCA	CAATCCGACGCTTTAGTCCA	50.0	50.0	57.8	58.0	979 bp	1.0	78.3
trnG-UCC-trnS-GCU_p4	GGTCCATAGAACGAATCGCA	AATCCGACGCTTTAGTCCAC	50.0	50.0	57.8	58.0	978 bp	1.0	78.3
trnG-UCC-trnS-GCU_p5	GGGGTCCATAGAACGAATCG	ACGGAAAGAGAGGGATTCGA	55.0	50.0	57.8	58.2	1034 bp	1.0	77.7