Hotspot

atpF-atpA

LSC · 52604-52676 · 73 bp

Back to Zollernia

Pi 0.0137

MarkerSeek score 60.40

Primer available yes

Label atpF-atpA

Feature Metadata

label_name: atpF-atpA
feature_type: igs
region: LSC
length_bp: 73
variable_sites: 1
pi: 0.0137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpA_p1	TGTTTGGGGCGATGAAAGAA	CCGTCGCCTACTTGAAGTAC	45.0	55.0	58.0	58.1	218 bp	1.0	79.5
atpF-atpA_p2	TGTTTGGGGCGATGAAAGAA	GTCGCCTACTTGAAGTACGG	45.0	55.0	58.0	58.1	216 bp	1.0	79.5
atpF-atpA_p3	AGCCTTACAGGGAGCTCTAG	CCGTCGCCTACTTGAAGTAC	55.0	55.0	57.9	58.1	301 bp	1.0	78.9
atpF-atpA_p4	AGCCTTACAGGGAGCTCTAG	GTCGCCTACTTGAAGTACGG	55.0	55.0	57.9	58.1	299 bp	1.0	78.9
atpF-atpA_p5	ACAGGGAGCTCTAGGAACTC	GTCGCCTACTTGAAGTACGG	55.0	55.0	57.8	58.1	293 bp	1.0	78.3