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psbM-petN

LSC · 33212-34387 · 1176 bp

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Pi 0.0017

MarkerSeek score 59.70

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 1176
variable_sites: 2
pi: 0.0017
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGCTACTGCACTGTTCATTCT	TCTTGCTTGGGCTGCTTTAA	42.9	45.0	57.6	58.0	1331 bp	1.0	68.8
psbM-petN_p2	TGCTACTGCACTGTTCATTCT	AGTAAGTCTTGCTTGGGCTG	42.9	50.0	57.6	57.8	1337 bp	1.0	67.3
psbM-petN_p3	TGCTACTGCACTGTTCATTCT	ATGGGGAAGAAGTGGACTCT	42.9	50.0	57.6	57.6	1277 bp	1.0	66.1
psbM-petN_p4	TGCTACTGCACTGTTCATTCT	CCCTCGTAGTATGGGGAAGA	42.9	55.0	57.6	57.6	1287 bp	1.0	65.9
psbM-petN_p5	TGCTACTGCACTGTTCATTCT	TCCCTCGTAGTATGGGGAAG	42.9	55.0	57.6	57.6	1288 bp	1.0	65.9