Hotspot

trnT-GGU-trnE-UUC

LSC · 31139-31940 · 802 bp

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Pi 0.00125

MarkerSeek score 59.50

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 802
variable_sites: 1
pi: 0.00125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	GAACCGATGACTTACGCCTT	TTTCGTAGTACCCTACCCCC	50.0	55.0	58.0	57.8	943 bp	1.0	78.8
trnT-GGU-trnE-UUC_p2	CCATGGCGTTACTCTACCAC	TTTCGTAGTACCCTACCCCC	55.0	55.0	58.1	57.8	922 bp	1.0	78.3
trnT-GGU-trnE-UUC_p3	TGGCGTTACTCTACCACTGA	TTTCGTAGTACCCTACCCCC	50.0	55.0	58.1	57.8	919 bp	1.0	78.1
trnT-GGU-trnE-UUC_p4	GAACCGATGACTTACGCCTT	GCTGCCTCCTTGAAAGAGAG	50.0	55.0	58.0	58.3	905 bp	1.0	77.9
trnT-GGU-trnE-UUC_p5	GAACCGATGACTTACGCCTT	TGTCCTGAACCACTAGACGA	50.0	50.0	58.0	57.7	884 bp	1.0	77.8