Hotspot

trnG-GCC-psbZ

LSC · 25565-26315 · 751 bp

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Pi 0.00134
MarkerSeek score 48.70
Primer available yes
Label trnG-GCC-psbZ

Feature Metadata

label_name
trnG-GCC-psbZ
feature_type
igs
region
LSC
length_bp
751
variable_sites
1
pi
0.00134
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-psbZ_p1 CGTCTTCTCCTTGGCAAAGA TGGATTAGTCTTTCTAGTGGGT 50.0 40.9 57.8 55.9 839-844 bp 1.0 46.5
trnG-GCC-psbZ_p2 CGTCTTCTCCTTGGCAAAGA TTGGATTAGTCTTTCTAGTGGGT 50.0 39.1 57.8 56.6 840-845 bp 1.0 44.3
trnG-GCC-psbZ_p3 CGTCTTCTCCTTGGCAAAGA TGGATTAGTCTTTCTAGTGGG 50.0 42.9 57.8 54.1 839-844 bp 1.0 40.6
trnG-GCC-psbZ_p4 CGTCTTCTCCTTGGCAAAGA GGATTAGTCTTTCTAGTGGGT 50.0 42.9 57.8 54.1 838-843 bp 1.0 40.6
trnG-GCC-psbZ_p5 CGTCTTCTCCTTGGCAAAGA TTGGATTAGTCTTTCTAGTGGG 50.0 40.9 57.8 54.9 840-845 bp 1.0 40.6