Hotspot

ycf1

SSC · 130292-136036 · 5745 bp

Back to Ziziphus

Pi 0.0152

MarkerSeek score 49.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5745
variable_sites: 261
pi: 0.0152
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCAGGCCTCAATATCGACT	ATCACAACCCTCAGCCCTTA	50.0	50.0	57.9	58.3	695-701 bp	1.0	81.9
ycf1_p2	TCCAGGCCTCAATATCGACT	TATCACAACCCTCAGCCCTT	50.0	50.0	57.9	58.3	696-702 bp	1.0	81.9
ycf1_p3	TCCAGGCCTCAATATCGACT	TTATCACAACCCTCAGCCCT	50.0	50.0	57.9	58.3	697-703 bp	1.0	81.9
ycf1_p4	TGGGAAAAACCAACGTTCCA	ATCACAACCCTCAGCCCTTA	45.0	50.0	58.2	58.3	919-925 bp	0.6	63.8
ycf1_p5	TGGGAAAAACCAACGTTCCA	TATCACAACCCTCAGCCCTT	45.0	50.0	58.2	58.3	920-926 bp	0.6	63.8