Hotspot

ndhA

SSC · 125961-128346 · 2386 bp

Back to Ziziphus

Pi 0.0143

MarkerSeek score 54.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2386
variable_sites: 103
pi: 0.0143
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CCCGTACTGTTTGTTGACCA	AGCCCCAAAAGGAGAATTGG	50.0	50.0	58.0	58.1	2641-2923 bp	0.9	81.2
ndhA_p2	CCCGTACTGTTTGTTGACCA	CAGGCTCTGGAAGGAATTCC	50.0	55.0	58.0	58.0	2805-2875 bp	0.8	76.8
ndhA_p3	CCCGTACTGTTTGTTGACCA	GAAGGAGATTCGCTAGCTCG	50.0	55.0	58.0	58.0	2874-2944 bp	0.8	76.7
ndhA_p4	CAATCGACGACGGGTAGATC	AGCCCCAAAAGGAGAATTGG	55.0	50.0	58.0	58.1	2871-2907 bp	0.4	58.1
ndhA_p5	AATCGACGACGGGTAGATCT	AGCCCCAAAAGGAGAATTGG	50.0	50.0	58.0	58.1	2870-2906 bp	0.4	58.1