Hotspot

petD-rpoA

LSC · 82375-82573 · 199 bp

Back to Ziziphus

Pi 0.0218

MarkerSeek score 64.70

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 199
variable_sites: 14
pi: 0.0218
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TGGTTGGGTATTGGAGCAAC	ACAGTGAAGAAGACCTTATGA	50.0	38.1	58.1	53.9	346-365 bp	1.0	45.4
petD-rpoA_p2	GATTGGTACTGCAGTAGCCC	ACAGTGAAGAAGACCTTATGA	55.0	38.1	58.0	53.9	368-387 bp	1.0	45.3
petD-rpoA_p3	AGCCCTTTGGTTGGGTATTG	ACAGTGAAGAAGACCTTATGA	50.0	38.1	58.1	53.9	353-372 bp	1.0	45.3
petD-rpoA_p4	GATTGGTACTGCAGTAGCCC	ACAGTGAAGAAGACCTTATGAA	55.0	36.4	58.0	54.8	368-387 bp	1.0	45.3
petD-rpoA_p5	CCCATTTCGTCGTCCAGTAG	ACAGTGAAGAAGACCTTATGA	55.0	38.1	58.1	53.9	404-423 bp	1.0	44.8