Hotspot

accD-psaI

LSC · 62832-63737 · 906 bp

Back to Ziziphus

Pi 0.0265

MarkerSeek score 65.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 906
variable_sites: 62
pi: 0.0265
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TAGTGTGTGCTTCTGGAGGA	TTATGCTCGGAAGAAGCCAC	50.0	50.0	58.0	58.0	1536-1612 bp	1.0	87.5
accD-psaI_p2	GAACCAAATGCCTACGTTGC	GTTTTTCGAGACCCCGCTAT	50.0	50.0	58.0	58.0	1707-1786 bp	1.0	87.4
accD-psaI_p3	AGTATGGGATCGGTAGTGGG	TTATGCTCGGAAGAAGCCAC	55.0	50.0	58.0	58.0	1612-1688 bp	1.0	87.3
accD-psaI_p4	TAGTGTGTGCTTCTGGAGGA	GTTTTTCGAGACCCCGCTAT	50.0	50.0	58.0	58.0	1895-1974 bp	1.0	86.9
accD-psaI_p5	AGTATGGGATCGGTAGTGGG	GTTTTTCGAGACCCCGCTAT	55.0	50.0	58.0	58.0	1971-2050 bp	1.0	86.6