Hotspot

trnS-GGA-rps4

LSC · 48659-48953 · 295 bp

Back to Ziziphus

Pi 0.0215

MarkerSeek score 64.10

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 295
variable_sites: 17
pi: 0.0215
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	AGGCTTTTGTTTACCGAGGG	AACGCTTCACCCATTTCAAT	50.0	40.0	58.1	56.2	444-450 bp	1.0	69.1
trnS-GGA-rps4_p2	GGCTTTTGTTTACCGAGGGT	AACGCTTCACCCATTTCAAT	50.0	40.0	58.4	56.2	443-449 bp	1.0	66.7
trnS-GGA-rps4_p3	ATGTAGGAGAGATGGCCGAG	AACGCTTCACCCATTTCAAT	55.0	40.0	58.4	56.2	496-502 bp	1.0	66.4
trnS-GGA-rps4_p4	AGGCTTTTGTTTACCGAGGG	TAACGCTTCACCCATTTCAA	50.0	40.0	58.1	55.6	445-451 bp	1.0	63.9
trnS-GGA-rps4_p5	AGGCTTTTGTTTACCGAGGG	TTAACGCTTCACCCATTTCA	50.0	40.0	58.1	55.6	446-452 bp	1.0	63.9