Hotspot

psbZ-trnG-GCC

LSC · 38762-39327 · 566 bp

Back to Ziziphus

Pi 0.0259

MarkerSeek score 65.20

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 566
variable_sites: 37
pi: 0.0259
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	AATCGAACCCGCATCTTCTC	50.0	50.0	57.8	58.1	648-734 bp	1.0	86.2
psbZ-trnG-GCC_p2	TTGCTTCTCCTGATGGTTGG	GAATCGAACCCGCATCTTCT	50.0	50.0	57.8	58.1	649-735 bp	1.0	86.2
psbZ-trnG-GCC_p3	TGGGCGGGTAACAAAAATGT	AATCGAACCCGCATCTTCTC	45.0	50.0	58.3	58.1	631-717 bp	1.0	85.6
psbZ-trnG-GCC_p4	TGGGCGGGTAACAAAAATGT	GAATCGAACCCGCATCTTCT	45.0	50.0	58.3	58.1	632-718 bp	1.0	85.6
psbZ-trnG-GCC_p5	TTGCTTCTCCTGATGGTTGG	GAACCCGCATCTTCTCCTTG	50.0	55.0	57.8	58.6	644-730 bp	1.0	81.7