Hotspot

rps2-rpoC2

LSC · 17674-17896 · 223 bp

Back to Ziziphus

Pi 0.0434

MarkerSeek score 72.20

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 223
variable_sites: 20
pi: 0.0434
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	GTGCCATTCGAGGATTCCAT	TGCACCACAGAGAATTATTTGA	50.0	36.4	58.0	56.0	407-412 bp	1.0	52.4
rps2-rpoC2_p2	AACTCCTGCTCCCATCATCT	TGCACCACAGAGAATTATTTGA	50.0	36.4	58.1	56.0	364-369 bp	1.0	52.3
rps2-rpoC2_p3	TGCTCCCATCATCTCTTCCA	TGCACCACAGAGAATTATTTGA	50.0	36.4	58.1	56.0	358-363 bp	1.0	52.3
rps2-rpoC2_p4	AAGGTGCCATTCGAGGATTC	TGCACCACAGAGAATTATTTGA	50.0	36.4	58.0	56.0	410-415 bp	1.0	52.2
rps2-rpoC2_p5	TCCTGCTCCCATCATCTCTT	TGCACCACAGAGAATTATTTGA	50.0	36.4	57.8	56.0	361-366 bp	1.0	51.7