Hotspot

atpH-atpI

LSC · 14911-16003 · 1093 bp

Back to Ziziphus

Pi 0.0192

MarkerSeek score 72.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1093
variable_sites: 58
pi: 0.0192
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	GCGAATCCATGGAAGGTCAT	45.0	50.0	58.0	58.0	1136-1151 bp	1.0	85.6
atpH-atpI_p2	AATAGAAGCAAGCCCGACAG	GCGAATCCATGGAAGGTCAT	50.0	50.0	58.0	58.0	1170-1185 bp	1.0	85.5
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACCTTAGCTGCG	45.0	45.0	58.0	57.9	1166-1181 bp	1.0	84.6
atpH-atpI_p4	AGCCAATCCAGCAGCAATAA	GCGAATCCATGGAAGGTCAT	45.0	50.0	57.9	58.0	1152-1167 bp	1.0	84.6
atpH-atpI_p5	AATAGAAGCAAGCCCGACAG	TTTTTGCAACCTTAGCTGCG	50.0	45.0	58.0	57.9	1200-1215 bp	1.0	84.5