Hotspot

atpF-atpH

LSC · 14192-14664 · 473 bp

Back to Ziziphus

Pi 0.0204

MarkerSeek score 67.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 473
variable_sites: 26
pi: 0.0204
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	GTTGTGGCATTAGCGCTTTT	50.0	45.0	57.9	57.9	561-584 bp	1.0	86.0
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	TGGACTGGTTGTGGCATTAG	50.0	50.0	57.9	57.8	568-591 bp	1.0	84.8
atpF-atpH_p3	TGGCCAATAACCCAAGGAAA	GTTGTGGCATTAGCGCTTTT	45.0	45.0	57.3	57.9	537-560 bp	1.0	81.6
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	TTTATGGACTGGTTGTGGCA	50.0	45.0	57.9	57.3	572-595 bp	1.0	81.0
atpF-atpH_p5	TGGCCAATAACCCAAGGAAA	TGGACTGGTTGTGGCATTAG	45.0	50.0	57.3	57.8	544-567 bp	1.0	80.4