Hotspot

trnS-GCU-trnG-UCC

LSC · 8621-9695 · 1075 bp

Back to Ziziphus

Pi 0.031

MarkerSeek score 63.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1075
variable_sites: 93
pi: 0.031
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ACGGAAAGAGAGGGATTCGA	TACCACTAAACTATACCCGC	50.0	45.0	58.2	54.0	1109-1266 bp	1.0	57.1
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	TTACCACTAAACTATACCCGC	50.0	42.9	58.2	54.9	1110-1267 bp	1.0	56.0
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	TTTACCACTAAACTATACCCGC	50.0	40.9	58.2	55.6	1111-1268 bp	1.0	54.3
trnS-GCU-trnG-UCC_p4	CTTTCGTCCACTCAGCCATC	TACCACTAAACTATACCCGC	55.0	45.0	58.6	54.0	1045-1202 bp	1.0	53.5
trnS-GCU-trnG-UCC_p5	AACGGAAAGAGAGGGATTCG	TACCACTAAACTATACCCGC	50.0	45.0	57.3	54.0	1110-1267 bp	1.0	52.9