Hotspot

trnH-GUG-psbA

LSC · 76-420 · 345 bp

Back to Ziziphus

Pi 0.0353

MarkerSeek score 67.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 345
variable_sites: 32
pi: 0.0353
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CGAAGTTCCGTCTACAAACG	55.0	50.0	58.1	56.9	352-391 bp	1.0	81.0
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CGAAGTTCCGTCTACAAACG	50.0	50.0	57.9	56.9	366-405 bp	1.0	80.7
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CGAAGTTCCGTCTACAAACG	50.0	50.0	57.9	56.9	365-404 bp	1.0	80.7
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CGAAGTTCCGTCTACAAACG	50.0	50.0	57.9	56.9	367-406 bp	1.0	80.7
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	TCGAAGTTCCGTCTACAAACG	55.0	47.6	58.1	58.3	353-392 bp	1.0	79.1