Hotspot

ycf1

SSC · 121686-127301 · 5616 bp

Back to Ziziphora

Pi 0.0068

MarkerSeek score 45.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5616
variable_sites: 76
pi: 0.0068
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGATTTCGGATTGTACTTGT	AGAAGTCTATAAATCGAGCGAACT	35.0	37.5	52.5	57.6	168 bp	1.0	42.9
ycf1_p2	TGATTTCGGATTGTACTTGTT	AGAAGTCTATAAATCGAGCGAACT	33.3	37.5	53.5	57.6	168 bp	1.0	42.9
ycf1_p3	TGATTTCGGATTGTACTTGT	AGAGAAGTCTATAAATCGAGCGA	35.0	39.1	52.5	56.7	170 bp	1.0	42.8
ycf1_p4	TGATTTCGGATTGTACTTGTT	AGAGAAGTCTATAAATCGAGCGA	33.3	39.1	53.5	56.7	170 bp	1.0	42.8
ycf1_p5	TTGATTTCGGATTGTACTTGT	AGAGAAGTCTATAAATCGAGCGA	33.3	39.1	53.5	56.7	171 bp	1.0	42.8