Hotspot

ndhG-ndhI

SSC · 116660-117046 · 387 bp

Back to Ziziphora

Pi 0.011

MarkerSeek score 52.00

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 387
variable_sites: 8
pi: 0.011
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TCCCAATGAAAAGGCCGAAT	ATTTCTTTAGGCCGGTTACC	45.0	45.0	57.8	55.4	591-597 bp	1.0	59.8
ndhG-ndhI_p2	TCCCAATGAAAAGGCCGAAT	TCAAATTTCTTTAGGCCGGT	45.0	40.0	57.8	55.2	595-601 bp	1.0	58.3
ndhG-ndhI_p3	TCCCAATGAAAAGGCCGAAT	AATTTCTTTAGGCCGGTTACC	45.0	42.9	57.8	56.2	592-598 bp	1.0	58.1
ndhG-ndhI_p4	TCCCAATGAAAAGGCCGAAT	TCAAATTTCTTTAGGCCGGTT	45.0	38.1	57.8	56.0	595-601 bp	1.0	56.8
ndhG-ndhI_p5	TCCCAATGAAAAGGCCGAAT	AAATTTCTTTAGGCCGGTTACC	45.0	40.9	57.8	56.9	593-599 bp	1.0	56.0