Hotspot

rpl32-trnL-UAG

SSC · 111326-112085 · 760 bp

Back to Ziziphora

Pi 0.00921

MarkerSeek score 52.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 760
variable_sites: 15
pi: 0.00921
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TCTCTTTCTACTGGGACTTCA	GATGATATGCCATGCCGCTA	42.9	50.0	55.4	58.0	911-939 bp	1.0	53.7
rpl32-trnL-UAG_p2	TCTCTTTCTACTGGGACTTCA	TCCTAAGAGCAGCGTGTCTA	42.9	50.0	55.4	57.9	858-886 bp	1.0	53.1
rpl32-trnL-UAG_p3	TCTCTTTCTACTGGGACTTCA	AGCAGCGTGTCTACCAATTT	42.9	45.0	55.4	57.8	851-879 bp	1.0	52.7
rpl32-trnL-UAG_p4	TCTCTTTCTACTGGGACTTCA	CCTAAGAGCAGCGTGTCTAC	42.9	55.0	55.4	57.8	857-885 bp	1.0	52.6
rpl32-trnL-UAG_p5	TCTCTTTCTACTGGGACTTCA	CTAAGAGCAGCGTGTCTACC	42.9	55.0	55.4	57.8	856-884 bp	1.0	52.6