Hotspot

rpl20-rps12

LSC · 67956-68760 · 805 bp

Back to Ziziphora

Pi 0.00174

MarkerSeek score 51.70

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 805
variable_sites: 3
pi: 0.00174
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGAGCTATATATCCGCGT	ACAGCCCATTCGAAATGTCA	50.0	45.0	58.0	57.8	918-927 bp	1.0	79.5
rpl20-rps12_p2	TCCGAGCTATATATCCGCGT	ACAAGACAGCCCATTCGAAA	50.0	45.0	58.0	57.7	923-932 bp	1.0	78.9
rpl20-rps12_p3	ACCCTCGAAAACTTGATGCA	ACAGCCCATTCGAAATGTCA	45.0	45.0	57.7	57.8	963-972 bp	1.0	77.6
rpl20-rps12_p4	TCCGAGCTATATATCCGCGT	AGACAGCCCATTCGAAATGT	50.0	45.0	58.0	57.5	920-929 bp	1.0	77.1
rpl20-rps12_p5	ACCCTCGAAAACTTGATGCA	ACAAGACAGCCCATTCGAAA	45.0	45.0	57.7	57.7	968-977 bp	1.0	77.0