Hotspot

ndhC-trnV-UAC

LSC · 50068-51031 · 964 bp

Back to Ziziphora

Pi 0.00692

MarkerSeek score 48.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 964
variable_sites: 14
pi: 0.00692
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTTTCCTAAGCGGGGCTAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.6	57.8	1103-1109 bp	1.0	76.6
ndhC-trnV-UAC_p2	AAGCGGGGCTAAAATTCCAG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.5	57.8	1096-1102 bp	1.0	75.7
ndhC-trnV-UAC_p3	GCGGGGCTAAAATTCCAGAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.5	57.8	1094-1100 bp	1.0	75.6
ndhC-trnV-UAC_p4	TTTCTCCGGCCCTTTCCTAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.6	57.8	1114-1120 bp	1.0	74.8
ndhC-trnV-UAC_p5	CTTTCCTAAGCGGGGCTAAA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.6	57.5	1102-1108 bp	1.0	73.8