Hotspot

trnT-GGU-psbD

LSC · 30833-32203 · 1371 bp

Back to Ziziphora

Pi 0.00472

MarkerSeek score 56.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1371
variable_sites: 14
pi: 0.00472
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	ACAGAGTGACTCGTTCAGGA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.0	58.1	1579-1599 bp	1.0	79.7
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1531-1551 bp	1.0	79.4
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1534-1554 bp	1.0	79.2
trnT-GGU-psbD_p4	ACAGAGTGACTCGTTCAGGA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.0	58.2	1569-1589 bp	1.0	78.7
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1521-1541 bp	1.0	78.3