Hotspot

petN-psbM

LSC · 28196-29153 · 958 bp

Back to Ziziphora

Pi 0.00682

MarkerSeek score 58.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 958
variable_sites: 13
pi: 0.00682
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACCGCTTTTCTACT	50.0	42.9	58.2	56.4	1094-1099 bp	1.0	59.4
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TCATTCTAGTTCCTACCGCT	50.0	45.0	58.2	55.4	1101-1106 bp	1.0	59.2
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	TCATTCTAGTTCCTACCGCTT	50.0	42.9	58.2	56.2	1101-1106 bp	1.0	57.7
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TTCATTCTAGTTCCTACCGCT	50.0	42.9	58.2	56.2	1102-1107 bp	1.0	57.7
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACCGCTTTTCTACTT	50.0	40.9	58.2	57.1	1094-1099 bp	1.0	57.2