Hotspot

rpoB-trnC-GCA

LSC · 26773-27874 · 1102 bp

Back to Ziziphora

Pi 0.00684

MarkerSeek score 65.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1102
variable_sites: 14
pi: 0.00684
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AGTTCTTCTGCCAAACCCTG	TTTTGGTGATTCAGGCGACA	50.0	45.0	58.0	58.0	1289-1331 bp	1.0	81.5
rpoB-trnC-GCA_p2	GTTCTTCTGCCAAACCCTGA	TTTTGGTGATTCAGGCGACA	50.0	45.0	58.0	58.0	1288-1330 bp	1.0	81.5
rpoB-trnC-GCA_p3	AGTTCTTCTGCCAAACCCTG	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1268-1310 bp	1.0	81.1
rpoB-trnC-GCA_p4	GTTCTTCTGCCAAACCCTGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1267-1309 bp	1.0	81.1
rpoB-trnC-GCA_p5	GCCAAACCCTGATCGATGAA	TTTTGGTGATTCAGGCGACA	50.0	45.0	58.2	58.0	1280-1322 bp	1.0	79.6