Hotspot

rps2-rpoC2

LSC · 16142-16368 · 227 bp

Back to Ziziphora

Pi 0.0106

MarkerSeek score 65.30

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 227
variable_sites: 5
pi: 0.0106
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	CATGGCCAAAATGAACTCCC	TTCGAGGGGGAAATGAGAGA	50.0	50.0	57.3	57.8	400-414 bp	1.0	76.3
rps2-rpoC2_p2	CCATGGCCAAAATGAACTCC	TTCGAGGGGGAAATGAGAGA	50.0	50.0	57.3	57.8	401-415 bp	1.0	76.3
rps2-rpoC2_p3	ATGGCCAAAATGAACTCCCG	TTCGAGGGGGAAATGAGAGA	50.0	50.0	58.8	57.8	399-413 bp	1.0	75.2
rps2-rpoC2_p4	CATGGCCAAAATGAACTCCC	TCGAGGGGGAAATGAGAGAT	50.0	50.0	57.3	57.5	399-413 bp	1.0	74.5
rps2-rpoC2_p5	CCATGGCCAAAATGAACTCC	TCGAGGGGGAAATGAGAGAT	50.0	50.0	57.3	57.5	400-414 bp	1.0	74.5