Hotspot

psbI-trnS-GCU

LSC · 7988-8118 · 131 bp

Back to Ziziphora

Pi 0.0112

MarkerSeek score 54.40

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 131
variable_sites: 3
pi: 0.0112
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	ATCTAATGATCCGGGGCGTA	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.1	58.0	239-279 bp	1.0	82.3
psbI-trnS-GCU_p2	TATCTAATGATCCGGGGCGT	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.1	58.0	240-280 bp	1.0	82.3
psbI-trnS-GCU_p3	ATCTAATGATCCGGGGCGTA	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.1	58.0	240-280 bp	1.0	82.2
psbI-trnS-GCU_p4	TATCTAATGATCCGGGGCGT	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.1	58.0	241-281 bp	1.0	82.2
psbI-trnS-GCU_p5	ATCTAATGATCCGGGGCGTA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	58.1	58.1	258-298 bp	1.0	81.2