Hotspot

trnH-GUG-psbA

LSC · 86-399 · 314 bp

Back to Ziziphora

Pi 0.0214

MarkerSeek score 53.10

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 314
variable_sites: 13
pi: 0.0214
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CAACCGTGCTAACCTTGGTA	55.0	50.0	58.1	57.8	432-450 bp	1.0	81.1
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	ATCAACCGTGCTAACCTTGG	55.0	50.0	58.1	58.2	434-452 bp	1.0	81.0
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	446-464 bp	1.0	80.9
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	445-463 bp	1.0	80.9
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	447-465 bp	1.0	80.9