Hotspot

rrn16-trnI-GAU

IRb · 94239-94547 · 309 bp

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Pi 0.00647
MarkerSeek score 57.20
Primer available yes
Label rrn16-trnI-GAU

Feature Metadata

label_name
rrn16-trnI-GAU
feature_type
igs
region
IRb
length_bp
309
variable_sites
3
pi
0.00647
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn16-trnI-GAU_p1 TAAGGAGGGGGATGCCTAAG CTATCCATGTGGCTGAGAGC 55.0 55.0 57.9 57.8 484-485 bp 1.0 78.4
rrn16-trnI-GAU_p2 TAAGGAGGGGGATGCCTAAG CGCTGATGAGCACATTGAAC 55.0 50.0 57.9 57.8 503-504 bp 1.0 78.3
rrn16-trnI-GAU_p3 TAAGGAGGGGGATGCCTAAG GGCACAACGACGCAATTATC 55.0 50.0 57.9 58.2 452-453 bp 1.0 78.2
rrn16-trnI-GAU_p4 GTAAGGAGGGGGATGCCTAA CTATCCATGTGGCTGAGAGC 55.0 55.0 58.2 57.8 485-486 bp 1.0 77.9
rrn16-trnI-GAU_p5 GTAAGGAGGGGGATGCCTAA CGCTGATGAGCACATTGAAC 55.0 50.0 58.2 57.8 504-505 bp 1.0 77.8