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psbT-psbN

LSC · 72082-72129 · 48 bp

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Pi 0.0833

MarkerSeek score 60.50

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 48
variable_sites: 6
pi: 0.0833
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TCTTCTTCCGAGAACCACCT	TCGCCATCTCCATATCTGGT	50.0	50.0	58.0	58.0	208 bp	1.0	82.5
psbT-psbN_p2	ACTACAAGGAGACAGCCAGT	TCGCCATCTCCATATCTGGT	50.0	50.0	58.0	58.0	457 bp	1.0	81.0
psbT-psbN_p3	TCTTCTTCCGAGAACCACCT	TAAACTACCTGCGCGAGTTC	50.0	50.0	58.0	58.0	396 bp	0.666667	66.5
psbT-psbN_p4	ACTACAAGGAGACAGCCAGT	TAAACTACCTGCGCGAGTTC	50.0	50.0	58.0	58.0	645 bp	0.666667	66.4
psbT-psbN_p5	TTCCGAGAACCACCTAAGGT	TAAACTACCTGCGCGAGTTC	50.0	50.0	58.0	58.0	391 bp	0.666667	66.4