Hotspot

atpB-rbcL

LSC · 54484-55246 · 763 bp

Back to Zizania

Pi 0.01

MarkerSeek score 53.40

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 763
variable_sites: 11
pi: 0.01
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGACGAGAAGTAGTAGGATTGG	ACTCCGGGGTGTAGTAAGTC	50.0	55.0	57.4	58.2	875-896 bp	1.0	59.5
atpB-rbcL_p2	GGGACGAGAAGTAGTAGGATTG	ACTCCGGGGTGTAGTAAGTC	50.0	55.0	57.4	58.2	876-897 bp	1.0	59.5
atpB-rbcL_p3	GGACGAGAAGTAGTAGGATTGG	CTCCGGGGTGTAGTAAGTCA	50.0	55.0	57.4	58.2	874-895 bp	1.0	59.4
atpB-rbcL_p4	GGGACGAGAAGTAGTAGGATTG	CTCCGGGGTGTAGTAAGTCA	50.0	55.0	57.4	58.2	875-896 bp	1.0	59.4
atpB-rbcL_p5	GGACGAGAAGTAGTAGGATTGG	CGTACTCCGGGGTGTAGTAA	50.0	55.0	57.4	58.2	878-899 bp	1.0	58.7