Hotspot

ndhC-trnV-UAC

LSC · 50774-51541 · 768 bp

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Pi 0.0122
MarkerSeek score 47.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
768
variable_sites
14
pi
0.0122
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GAACCGGAGCTAAAAGTGCT GGTTCGAATCCGTATAGCCC 50.0 55.0 58.2 57.9 893-1007 bp 1.0 79.4
ndhC-trnV-UAC_p2 CCGGAGCTAAAAGTGCTGAA GGTTCGAATCCGTATAGCCC 50.0 55.0 58.2 57.9 890-1004 bp 1.0 79.4
ndhC-trnV-UAC_p3 GAACCGGAGCTAAAAGTGCT CGGTTCGAATCCGTATAGCC 50.0 55.0 58.2 58.3 894-1008 bp 1.0 77.6
ndhC-trnV-UAC_p4 CCGGAGCTAAAAGTGCTGAA CGGTTCGAATCCGTATAGCC 50.0 55.0 58.2 58.3 891-1005 bp 1.0 77.6
ndhC-trnV-UAC_p5 AACCGGAGCTAAAAGTGCTG GGTTCGAATCCGTATAGCCC 50.0 55.0 58.5 57.9 892-1006 bp 1.0 77.1