Hotspot

trnY-GUA-trnD-GUC

LSC · 15968-16304 · 337 bp

Back to Zizania

Pi 0.0168

MarkerSeek score 62.90

Primer available yes

Label trnY-GUA-trnD-GUC

Feature Metadata

label_name: trnY-GUA-trnD-GUC
feature_type: igs
region: LSC
length_bp: 337
variable_sites: 8
pi: 0.0168
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnD-GUC_p1	AATTCTTCCTGGGTCGATGC	CGGTGCTCTGACCAATTGAA	50.0	50.0	58.0	58.5	398-463 bp	1.0	81.3
trnY-GUA-trnD-GUC_p2	GGGGACGGACTGTAAATTCG	CGGTGCTCTGACCAATTGAA	55.0	50.0	58.4	58.5	365-430 bp	1.0	78.9
trnY-GUA-trnD-GUC_p3	GGGACGGACTGTAAATTCGT	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.6	58.5	364-429 bp	1.0	78.8
trnY-GUA-trnD-GUC_p4	AATTCTTCCTGGGTCGATGC	GGTGCTCTGACCAATTGAAC	50.0	50.0	58.0	57.0	397-462 bp	1.0	77.1
trnY-GUA-trnD-GUC_p5	TGGGGACGGACTGTAAATTC	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.2	58.5	366-431 bp	1.0	75.6