Hotspot

trnG-UCC-trnT-GGU

LSC · 13591-15146 · 1556 bp

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Pi 0.00857
MarkerSeek score 52.00
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1556
variable_sites
20
pi
0.00857
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 TTTCCACCGAGCTGAAACAA ACGTCTTACCACGGCATTAC 45.0 50.0 58.0 58.0 2137-2140 bp 1.0 81.4
trnG-UCC-trnT-GGU_p2 TTTCCACCGAGCTGAAACAA CACGTCTTACCACGGCATTA 45.0 50.0 58.0 58.0 2138-2141 bp 1.0 81.4
trnG-UCC-trnT-GGU_p3 CATCGTTAGCTTGGAAGGCT ACGTCTTACCACGGCATTAC 50.0 50.0 58.0 58.0 2418-2421 bp 1.0 81.3
trnG-UCC-trnT-GGU_p4 CATCGTTAGCTTGGAAGGCT CACGTCTTACCACGGCATTA 50.0 50.0 58.0 58.0 2419-2422 bp 1.0 81.3
trnG-UCC-trnT-GGU_p5 CGTTAGCTTGGAAGGCTAGG ACGTCTTACCACGGCATTAC 55.0 50.0 58.1 58.0 2415-2418 bp 1.0 81.1