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psbK-psbI

LSC · 6973-7364 · 392 bp

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Pi 0.0171

MarkerSeek score 54.50

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 392
variable_sites: 10
pi: 0.0171
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ATTTTTACTCGTCACGCCCA	50.0	45.0	57.9	57.8	536-539 bp	1.0	79.8
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	ATTTTTACTCGTCACGCCCA	45.0	45.0	58.3	57.8	533-536 bp	1.0	77.8
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	ATTTTTACTCGTCACGCCCA	45.0	45.0	58.3	57.8	534-537 bp	1.0	77.8
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	ATTTTTACTCGTCACGCCCA	45.0	45.0	58.3	57.8	535-538 bp	1.0	77.8
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	TTTTTACTCGTCACGCCCAG	50.0	50.0	57.9	58.5	535-538 bp	1.0	77.2