Hotspot

trnK-UUU-rps16

LSC · 3941-4513 · 573 bp

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Pi 0.014

MarkerSeek score 55.30

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 573
variable_sites: 12
pi: 0.014
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	ACCAAGAGTCTGGACGAACT	AGAAAAAGAAAGAACTTTGAGT	50.0	27.3	58.3	52.1	689-695 bp	1.0	42.0
trnK-UUU-rps16_p2	ATCAGTCGCGGTCTTCTAGA	AGAAAAAGAAAGAACTTTGAGT	50.0	27.3	58.0	52.1	713-719 bp	1.0	41.9
trnK-UUU-rps16_p3	ATCAGTCGCGGTCTTCTAGA	AAGAAAAAGAAAGAACTTTGAGT	50.0	26.1	58.0	53.0	714-720 bp	1.0	41.9
trnK-UUU-rps16_p4	ATCAGTCGCGGTCTTCTAGA	AGAAAAAGAAAGAACTTTGAGTT	50.0	26.1	58.0	53.0	713-719 bp	1.0	41.9
trnK-UUU-rps16_p5	ATCAGTCGCGGTCTTCTAGA	AAGAAAAAGAAAGAACTTTGAGTT	50.0	25.0	58.0	53.8	714-720 bp	1.0	41.9