Hotspot

rpl32-trnL-UAG

SSC · 121104-121987 · 884 bp

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Pi 0.02

MarkerSeek score 63.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 884
variable_sites: 84
pi: 0.02
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ACCGGACGTTCAAAAAGTTT	TCCTAAGAGCAGCGTGTCTA	40.0	50.0	56.4	57.9	731-1035 bp	1.0	77.3
rpl32-trnL-UAG_p2	ACCGGACGTTCAAAAAGTTT	AGCAGCGTGTCTACCAATTT	40.0	45.0	56.4	57.8	724-1028 bp	1.0	76.9
rpl32-trnL-UAG_p3	ACCGGACGTTCAAAAAGTTT	CTAAGAGCAGCGTGTCTACC	40.0	55.0	56.4	57.8	729-1033 bp	1.0	76.8
rpl32-trnL-UAG_p4	ACCGGACGTTCAAAAAGTTT	CCTAAGAGCAGCGTGTCTAC	40.0	55.0	56.4	57.8	730-1034 bp	1.0	76.7
rpl32-trnL-UAG_p5	ACCGGACGTTCAAAAAGTTT	GAGCAGCGTGTCTACCAATT	40.0	50.0	56.4	58.3	725-1029 bp	1.0	76.2