Hotspot

trnI-CAU-ycf2

IRb · 90885-91063 · 179 bp

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Pi 0.0635
MarkerSeek score 79.80
Primer available yes
Label trnI-CAU-ycf2

Feature Metadata

label_name
trnI-CAU-ycf2
feature_type
igs
region
IRb
length_bp
179
variable_sites
66
pi
0.0635
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-CAU-ycf2_p1 GTTGGGCGCTTTAACCATTC CTCAATTCGACGATCCAGGA 50.0 50.0 58.0 57.1 202-499 bp 1.0 85.0
trnI-CAU-ycf2_p2 GTTGGGCGCTTTAACCATTC TCTCAATTCGACGATCCAGG 50.0 50.0 58.0 57.1 203-500 bp 1.0 85.0
trnI-CAU-ycf2_p3 AGTTGGGCGCTTTAACCATT CTCAATTCGACGATCCAGGA 45.0 50.0 58.4 57.1 203-500 bp 1.0 82.1
trnI-CAU-ycf2_p4 AGTTGGGCGCTTTAACCATT TCTCAATTCGACGATCCAGG 45.0 50.0 58.4 57.1 204-501 bp 1.0 82.1
trnI-CAU-ycf2_p5 TTGGGCGCTTTAACCATTCA CTCAATTCGACGATCCAGGA 45.0 50.0 58.4 57.1 201-498 bp 1.0 82.0