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psbT-psbN

LSC · 78255-78318 · 64 bp

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Pi 0.0712

MarkerSeek score 77.20

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 14
pi: 0.0712
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TTTTCGAGAACCGCCTAAGG	ATCCATTCGAAGAACACGGG	50.0	50.0	57.9	58.0	127-133 bp	1.0	90.7
psbT-psbN_p2	ACCGCCTAAGGTTCCAACTA	ATCCATTCGAAGAACACGGG	50.0	50.0	58.4	58.0	118-124 bp	1.0	88.9
psbT-psbN_p3	TTTTCGAGAACCGCCTAAGG	TTCGAAGAACACGGGGACTA	50.0	50.0	57.9	58.4	122-128 bp	1.0	88.0
psbT-psbN_p4	TTTTCGAGAACCGCCTAAGG	GGGTACGCCTTATATACCGC	50.0	55.0	57.9	57.7	176-182 bp	0.956522	84.5
psbT-psbN_p5	ACCGCCTAAGGTTCCAACTA	GGGTACGCCTTATATACCGC	50.0	55.0	58.4	57.7	167-173 bp	0.956522	82.9