Hotspot

petA-psbJ

LSC · 66723-67605 · 883 bp

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Pi 0.0184

MarkerSeek score 69.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 883
variable_sites: 70
pi: 0.0184
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CATTACGGGTCCAAGGTCTT	TCTGGGTTGGGTTCATCTCT	50.0	50.0	57.2	58.0	962-1035 bp	1.0	83.1
petA-psbJ_p2	CATTACGGGTCCAAGGTCTT	TGGGTTGGGTTCATCTCTCT	50.0	50.0	57.2	58.0	960-1033 bp	1.0	83.1
petA-psbJ_p3	CATTACGGGTCCAAGGTCTT	CCTCTTTGGTTGGTGGGTAC	50.0	55.0	57.2	58.1	1037-1110 bp	1.0	82.4
petA-psbJ_p4	CATTACGGGTCCAAGGTCTT	TGGTGGGTACTGTAACTGGT	50.0	50.0	57.2	57.9	1027-1100 bp	1.0	82.4
petA-psbJ_p5	CATTACGGGTCCAAGGTCTT	TCCTCTTTGGTTGGTGGGTA	50.0	50.0	57.2	58.2	1038-1111 bp	1.0	81.8