Hotspot

trnfM-CAU-rps14

LSC · 39484-39636 · 153 bp

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Pi 0.0409

MarkerSeek score 65.70

Primer available yes

Label trnfM-CAU-rps14

Feature Metadata

label_name: trnfM-CAU-rps14
feature_type: igs
region: LSC
length_bp: 153
variable_sites: 23
pi: 0.0409
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-rps14_p1	AAGGTTATGAGCCTTGCGAG	TTACCGGGCGCAATAAGATC	50.0	50.0	58.0	58.1	224-240 bp	1.0	90.6
trnfM-CAU-rps14_p2	ACAGGATTTGAACCCGTGAC	TTACCGGGCGCAATAAGATC	50.0	50.0	58.1	58.1	247-263 bp	1.0	89.6
trnfM-CAU-rps14_p3	AAGGTTATGAGCCTTGCGAG	ATGTTTATTACCGGGCGCAA	50.0	45.0	58.0	58.2	231-247 bp	1.0	89.5
trnfM-CAU-rps14_p4	AAGGTTATGAGCCTTGCGAG	TGTTTATTACCGGGCGCAAT	50.0	45.0	58.0	58.2	230-246 bp	1.0	89.5
trnfM-CAU-rps14_p5	TTTGAACCCGTGACCTCAAG	TTACCGGGCGCAATAAGATC	50.0	50.0	58.0	58.1	241-257 bp	0.913043	86.8