Hotspot

trnT-GGU-psbD

LSC · 34417-35697 · 1281 bp

Back to Zingiber

Pi 0.0134
MarkerSeek score 63.30
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1281
variable_sites
115
pi
0.0134
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GGTCTCTGCGTAACCAATCA 55.0 50.0 58.1 57.6 1260-1418 bp 1.0 87.0
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GGTCTCTGCGTAACCAATCA 50.0 50.0 58.1 57.6 1263-1421 bp 1.0 86.9
trnT-GGU-psbD_p3 GGCGTAAGTCATCGGTTCAA GGTCTCTGCGTAACCAATCA 50.0 50.0 58.3 57.6 1237-1395 bp 1.0 85.4
trnT-GGU-psbD_p4 GGCCCTTTTAACTCAGTGGT GGTCTCTGCGTAACCAATCA 50.0 50.0 57.7 57.6 1275-1433 bp 1.0 85.2
trnT-GGU-psbD_p5 GTGGTAGAGTAACGCCATGG CGGTCTCTGCGTAACCAATC 55.0 55.0 58.1 58.7 1261-1419 bp 1.0 84.3