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atpH-atpI

LSC · 14285-15720 · 1436 bp

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Pi 0.0179

MarkerSeek score 74.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1436
variable_sites: 157
pi: 0.0179
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	ATTTTTGCAACCTTAGCCGC	45.0	45.0	58.0	57.9	656-1542 bp	0.956522	89.3
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	ATTTTTGCAACCTTAGCCGC	50.0	45.0	57.9	57.9	712-1598 bp	0.956522	88.7
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	ATTTTTGCAACCTTAGCCGC	45.0	45.0	58.2	57.9	657-1543 bp	0.956522	88.2
atpH-atpI_p4	GCAGTACCTTGACCAACTCC	ATTTTTGCAACCTTAGCCGC	55.0	45.0	58.2	57.9	716-1602 bp	0.956522	88.1
atpH-atpI_p5	TACCTTCTACAGCTTGGCCT	ATTTTTGCAACCTTAGCCGC	50.0	45.0	58.0	57.9	739-1625 bp	0.869565	85.7