Hotspot

rps16-trnQ-UUG

LSC · 5932-7342 · 1411 bp

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Pi 0.021
MarkerSeek score 68.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1411
variable_sites
167
pi
0.021
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCCGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1268-1527 bp 0.913043 87.7
rps16-trnQ-UUG_p2 GCCGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1266-1525 bp 0.913043 87.7
rps16-trnQ-UUG_p3 AATCCACAGCCGATCATGTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1274-1533 bp 0.913043 87.6
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1250-1509 bp 0.913043 86.8
rps16-trnQ-UUG_p5 CAGCCGATCATGTCCTTCAA CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.0 57.8 1272-1531 bp 0.913043 86.6