Hotspot

atpF

LSC · 11023-12384 · 1362 bp

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Pi 0.0237

MarkerSeek score 63.80

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1362
variable_sites: 47
pi: 0.0237
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CACTTGAAGTACGGTACCGG	ATTTATGGACTGGTCGTGGC	55.0	50.0	58.0	58.0	1842-1878 bp	1.0	86.4
atpF_p2	CCACTTGAAGTACGGTACCG	ATTTATGGACTGGTCGTGGC	55.0	50.0	58.0	58.0	1843-1879 bp	1.0	86.3
atpF_p3	GCTCGAAGGGTTCCCATTAG	ATTTATGGACTGGTCGTGGC	55.0	50.0	58.0	58.0	1754-1790 bp	1.0	86.2
atpF_p4	CACTTGAAGTACGGTACCGG	GAGACAACCAGAAGCAGAGG	55.0	55.0	58.0	57.9	1915-1951 bp	1.0	85.7
atpF_p5	CCCCTCCTTCCTGGAAAGTA	ATTTATGGACTGGTCGTGGC	55.0	50.0	58.0	58.0	2389-2425 bp	1.0	85.0